NHS ester activated Rhodamine derivative with 2 sulfonate groups
1 use to label a total of 7mg of IgG using typical conditions
DyLight 590-R2 NHS Ester
DyLight Orange-emitting Dyes are a family of labeling agents that provide bright fluorescent detection for imaging. Dyes can be selected based upon characteristic excitation and emission properties or relative hydrophilicity/hydrophobicity attributes. Dyes that contain a greater number of negatively charged sulfonates generally will have greater water solubility than dyes with fewer sulfonates. More hydrophobic dyes often provide better cell penetrating ability in vivo, while more hydrophilic dyes have less nonspecific binding potential. Each dye contains an amine-reactive NHS ester for simple modification of antibodies, proteins, peptides or other biomolecules through amide bond formation.
NHS ester reactive group–allows immediate labeling of antibodies, proteins, peptides and other amine-containing molecules through amide bond formation
Multiple solubility options –choose from hydrophilic to hydrophobic dyes to optimize the right dye label for the best performance in a given application
Criteria to consider when choosing a DyLight Orange-emitting Specialty Dye:
Excitation and emission wavelengths –choose the best dye to match the excitation and emission capabilities of your instrument
Water solubility–choose a dye based on its relative hydrophilicity, which directly correlates to the number of negatively-charged sulfonates it has on its core structure. More hydrophilic dyes are best at maintaining water solubility of a labeled antibody and limiting the nonspecific binding of the conjugate. More hydrophobic dyes often are best at penetrating tissues and cell membranes in vivo, meaning that dyes with fewer sulfonates may work best for some applications.
Imaging; Antibody labeling; Direct immunofluorescence staining; Flow cytometry; Fluorescence correlation spectroscopy; ELISA; Western blotting; Protein microarrays; Polymer labeling; Peptide labeling; Phalloidin labeling for actin staining; Staining in acidic media; Biofilm microorganism staining