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DpnI

Cut at Gm6A^TC sites with DpnI restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: MalI).

Brand:  Thermo Scientific ER1701

Code : 88

Additional Details : Weight : 0.00900kg

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Product Code. 10669500

  • £29.59 / 500 units


EU Stock 33
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Description

Description

Highlights

  • Superior quality – stringent quality control and industry leading manufacturing process
  • Convenient color-coded Five Buffer System
  • Includes universal Tango buffer for double-digestions
  • BSA premixed in reaction buffers
  • Wide selection of restriction endonuclease specificities

Applications

  • Molecular cloning
  • Restriction site mapping
  • Genotyping
  • Southern Blot
  • Restriction fragment length polymorphism (RFLP)
  • SNP

Note

DpnI requires the presence of N6-methyladenine within the recognition sequence to cleave DNA. DNA purified from a dam+ strain will be a substrate for DpnI. DpnI will only cleave fully-adenomethylated dam sites. Hemi-adenomethylated dam sites DpnI cleaves 60X more slowly. DpnI, Bsp143I, and MboI all recognize the same sequence, but have different methylation sensitivities and cleavage sites. Assayed using pBR322 DNA.

Specifications

Specifications

DpnI
10U/μL
37°C
500U
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