TCR DO11.10, PE-Cyanine7, clone: KJ1-26 (KJ126, KJI-26, KJI26), eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 25-5808-82
Code : NEW
Additional Details : Weight : 0.09500kg
DescriptionDescription: The KJ1-26 anti-idiotypic monoclonal antibody binds the T-cell receptor (TCR) expressed on DO11.10 and DO11.10.24 murine T cell hybridomas, but not on any other T cell hybridomas tested.The TCR expressed on DO11.10 recognizes an ovalbumin peptide in the context of I-A^d major histocompatibility molecules.The use of DO11.10 and KJ1-26 have proven to be instrumental in describing many of the intricate details of T cell immunity.Applications Reported: This KJ1-26 (KJ126, KJI-26, KJI26) antibody has been reported for use in flow cytometric analysis.Applications Tested: This KJ1-26 (KJ126, KJI-26, KJI26) antibody has been tested by flow cytometric analysis of DO11 cells. This can be used at less than or equal to 0.25μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100μL of cell sample + 100μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|D011.10, T-cell receptor DO11.10|
|IgG2a, kappa, kappa|
|Store at 2-8°C. Do not freeze. Light-sensitive material. This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling and experimental procedures.|
|KJ1-26 (KJ126, KJI-26, KJI26), KJI-26, KJI26)|
For Research Use Only.