TCR DO11.10 Monoclonal Antibody (KJ1-26 (KJ126, KJI-26, KJI26)), Biotin, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 13-5808-81

Product Code. 15279859

Description

Description: The KJ1-26 anti-idiotypic monoclonal antibody binds the T-cell receptor (TCR) expressed on DO11.10 and DO11.10.24 murine T cell hybridomas, but not on any other T cell hybridomas tested.The TCR expressed on DO11.10 recognizes an ovalbumin peptide in the context of I-A^{d^} major histocompatibility molecules.The use of DO11.10 and KJ1-26 have proven to be instrumental in describing many of the intricate details of T cell immunity. Applications Reported: This KJ1-26 (KJ126, KJI-26, KJI26) antibody has been reported for use in flow cytometric analysis. Applications Tested: This KJ1-26 (KJ126, KJI-26, KJI26) antibody has been tested by flow cytometric analysis of the DO11.10 T cell hybridoma. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Filtration: 0.2 μm post-manufacturing filtered.

TCR DO11 (DO11.10) is a receptor found on lymphocytes of DO11.10 transgenic mice and T-cell receptors of BALB/c-derived DO11.10 and DO11.10.24 T cell hybridomas. The DO11.10 TCR recognizes a C-terminal ovalbumin peptide (OVA 323-339) in the context of I-Ad major histocompatibility molecules. The use of antibodies against DO11.10 have proven to be instrumental in describing many of the intricate details of T cell immunity, including T cell activation, development, and function.

Specifications

• Antigen: TCR DO11.10
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: P01849
• Gene Symbols: Tcra
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 21473
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Form: Liquid

• Applications: Flow Cytometry
• Clone: KJ1-26 (KJ126, KJI-26, KJI26), KJI-26, KJI26)
• Conjugate: Biotin
• Gene: Tcra
• Gene Alias: D011.10; T cell receptor alpha chain; Tcra; Tcralpha
• Host Species: Mouse
• Quantity: 50 μg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG2a κ

For Research Use Only.