DLL1 (delta-like 1) Monoclonal Antibody (HMD1-5), Alexa Fluor™ 488, eBioscience™, Invitrogen™

Armenian Hamster Monoclonal Antibody

Brand:  Affymetrix eBioscience 53-5767-80

Product Code. 15342040

Description

Description: The HMD1-5 monoclonal antibody reacts with mouse Delta-like 1, one of five type I transmembrane proteins that serves as a Notch receptor ligand. Upon binding the Notch receptor (e.g. Notch 1-4), Delta-like 1 undergoes proteolytic cleavage, first by ADAM-family metalloproteases and then by gamma-secretase. The second cleavage event releases an intracellular fragment whose biological function remains controversial. Delta-like 1 is expressed by thymic stromal cells, as well as on macrophages, dendritic cells, and stromal cells in the spleen. This protein is also expressed in nonhematopoietic tissues such as the lung and brain. Delta-like 1 has been linked to B cell development and differentiation, especially marginal zone and plasma cells, regulation of splenic dendritic cells, and leukemogenesis. Applications Reported: This HMD1-5 antibody has been reported for use in flow cytometric analysis. Applications Tested: This HMD1-5 antibody has been tested by flow cytometric analysis on mouse splenocytes and transfected cells. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser. Filtration: 0.2 μm post-manufacturing filtered. Delta-like protein 1 (Dlk1, DLL1) is a transmembrane protein characterized by epidermal growth factor (EGF)-like repeats. DLL1, which is also known as preadipocyte factor 1 (pref-1) because of its ability to inhibit preadipocyte differentiation, regulates the differentiation of several other cell types. It is a ligand for Notch receptor. It blocks the differentiation of progenitor cells into the B-cell lineage while promoting the emergence of a population of cells with the characteristics of a T-cell/NK-cell precursor.

Specifications

• Antigen: DLL1 (delta-like 1)
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
• Gene Accession No.: Q61483
• Gene Symbols: Dll1
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 13388
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Form: Liquid

• Applications: Flow Cytometry
• Clone: HMD1-5
• Conjugate: Alexa Fluor 488
• Gene: Dll1
• Gene Alias: DLL1; delta-like 1 (Drosophila); DELTA1; DL1; Delta; OTTHUMP00000017690; delta (Drosophila)-like 1; delta-like 1; delta-like 1 protein
• Host Species: Armenian Hamster
• Quantity: 25 μg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG

For Research Use Only.