Invitrogen™ Dehydroepiandrosterone Sulfate (DHEA-S) Competitive ELISA Kit

Competitive ELISA

Brand:  Invitrogen™ EIADHEA

Product Code. 15880322

Includes: Pre-coated plate(s), standard, detector antibody, HRP conjugate, assay buffer, wash buffer, chromogen, stop solution, plate covers and lot-specific technical data sheet.

Description

The Dehydroepiandrosterone Sulfate (DHEA-S) ELISA quantitates DHEA-Se in serum,plasma,urine,saliva,dried fecal extracts or cell culture medium.
Principle of the method
The Dehydroepiandrosterone sulfate (DHEA-S) Competitive ELISA research-use-only kit uses a specifically generated antibody to measure Dehydroepiandrosterone sulfate (DHEA-S) in serum,plasma,urine,and saliva samples,and in fecal extracts. The kit will also quantitatively measure DHEA-S present in tissue culture media samples. A DHEA-S standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture sheep antibodies. A DHEA-S-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a polyclonal antibody to DHEA-S to each well. After a 2-hour incubation the plate is washed and substrate is added. The substrate reacts with the bound DHEA-S-peroxidase conjugate. After a short incubation,the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring at 450 nm.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity,specificity,precision,and lot-to-lot consistency. See manual for more information on validation.

Dehydroepiandrosterone sulfate (DHEA-S) is the major C19 steroid secreted by the adrenal cortex and a precursor in testosterone and estrogen biosynthesis. DHEA-S is identical across all species and so this kit should measure DHEA-S from all sources. It is produced by the addition of a sulfate group to dehydroepiandrosterone (DHEA), catalyzed by the sulfotransferase enzymes, SULT1A1 and SULT1E1, which also produce estrone sulfate from estrone. DHEA sulfate can also be back-converted to DHEA through the action of steroid sulfatase.

Specifications

• Assay Range: 96-60,000 pg/mL
• Description: Dehydroepiandrosterone sulfate (DHEA-S) Competitive ELISA Kit
• Conjugate: HRP
• Product Type: ELISA Kit
• Sample Type: Fecal Extract, Plasma, Saliva, Serum, Supernatant, Urine
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Intraassay CV: 7%
• Label or Dye: HRP
• Regulatory Status: RUO
• Sample Volume: Fecal Extract,50 μL; Plasma, 1 μL; Saliva,25 μL; Serum, 1 μL; Supernatant, 50 μL; Urine,25 μL
• Target: Chemical
• Total Assay Time: 2 hr. 30 min.

• Assay Sensitivity: 90.9 pg/mL
• Assay: Multiplex assay on the Luminex Platform
• Detection Method: Fluorescence
• Protein Family: Steroid hormone
• Sufficient For: 96 assays
• For Use With (Application): ELISA, Protein Assays, Aktivity Assays, Protein Biology, Reproductive Biology, Inflammation, Oxidative Stress, Metabolism, Cell Signaling, Epigenetics, Hematology, DNA Damage, Cancer Biology, Immunology, Neuroscience, Neurobiology, Steroid Hormone Detecti
• Interassay CV: 8.4%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, HRP-Conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Quantity: 96 Tests
• Research Discipline: Reproductive Biology
• Storage Requirements: -20°C
• Test Time: 1 hr. 20 min.