Cytokeratin 8, FITC, clone: LP3K, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 11-9938-80
Code : Z2
Additional Details : Weight : 0.25000kg
DescriptionDescription: This LP3K monoclonal antibody reacts with human cytokeratin 8 (K8), a 55-kDa member of the family of intermediate filament proteins. Cytokeratin 8 is a type II (or basic) keratin that is expressed in epithelial and carcinoma cells. Cytokeratins form the intracellular cytoskeletal network that maintains the integrity and stability of cells and tissues. More specifically, studies have demonstrated the involvement of cytokeratin 8 in protection against apoptosis, stress, or injury, as well as regulation of the cell cycle. This keratin is frequently co-expressed with cytokeratin 18, a type I (or acidic) keratin as a heterodimer. Although detected primarily in the cytoplasm of normal healthy cells, cytokeratin 8 has been found to localize to the plasma membrane in some tumor cells. Finally, cytokeratin 8 is phosphorylated on serine 73 in dividing cells.Applications Reported: This LP3K antibody has been reported for use in immunofluorescent microscopy.Applications Tested: This LP3K antibody has been tested by immunofluorescent staining of methanol-fixed MCF-7 cells. This can be used at less than or equal to 10μg/ml. It is recommended that the antibody be titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|Keratin, type II cytoskeletal 8, K-8, Keratin-8, K8|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
For Research Use Only.