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Cytokeratin 7, eFluor 615, clone: LP5K, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 42-9005-82

Code : Z2

Additional Details : Weight : 0.25000kg

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Product Code. 15391120

  • £147.88 / 100Āµg


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Description

Description

Description: This LP5K monoclonal antibody reacts with human cytokeratin 7 (K7), a 54-kDa type II (or basic) keratin expressed either alone or paired with cytokeratin 19 in simple epithelia, mesothelium, urothelium, and pseudostratified epithelium. Expression of cytokeratin 7 in the gastric foveolar, intestinal, and stratified squamous epithelia is extremely low or undetectable. Cytokeratins form the intracellular cytoskeletal network that maintains the integrity and stability of cells and tissues. In addition, most carcinomas express cytokeratin 7. The coordinated expression of this keratin with cytokeratin 20 is commonly used as a diagnostic marker for a variety of carcinomas.Applications Reported: This LP5K antibody has been reported for use in immunohistochemical and immunocytochemical staining.Applications Tested: This LP5K antibody has been tested immunocytochemistry on fixed and permeabilized MCF7 cells at less than or equal to 10 μg/mL. It is recommended that this antibody be carefully titrated for optimal performance in the assay of interest. This product has not been validated for flow cytometric analysis.Filter Recommendation: When using this eFluor® 615 antibody conjugate, we recommend a filter that will capture the 615 emission wavelength (for example, Excitation 560/55, 585LP, Emission 645/75). A standard Alexa Fluor® 594 filter is acceptable.Excitation: 595 nm; Emission: 615 nm.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

Cytokeratin 7
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P08729
Keratin, type II cytoskeletal 7, CK-7, Keratin-7, K7, Sarcolectin
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Human
ImmunocytochemistryĆ¾Immunohistochemistry
LP5K
eFluor 615
KRT7
Conjugated
KRT7
IgG2b
100μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
3855
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