Cutaneous Lymphocyte Antigen (CLA), eFluor 660, clone: HECA-452, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 50-9857-82
Code : NEW
Additional Details : Weight : 0.09500kg
DescriptionDescription: This HECA-452 monoclonal antibody reacts with human Cutaneous Lymphocyte Antigen (CLA). This cell surface glycoprotein is most highly expressed on T cells in the skin. Nevertheless, CLA can also be detected on some peripheral T and NK cells, as well as monocytes, neutrophils and CD1a+ dendritic cells. Studies have determined that this antibody recognizes sialofucosylated glycans and sialyl Lewis x (sLex). CLA, which binds E-selectin and endothelial cell-leukocyte adhesion molecule 1 (ELAM-1), is involved in T lymphocyte infiltration and adhesion at cutaneous sites of inflammation.The HECA-452 antibody has been reported to inhibit sLex binding to E-selectin; however, this property is still under debate in the literature.Applications Reported: This HECA-452 antibody has been reported for use in flow cytometric analysis, western blotting, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections.Applications Tested: This HECA-452 antibody has been tested by flow cytometric analysis on normal human peripheral blood cells. This HECA-452 antibody has also been tested by immunhistochemistry on FFPE human tissue and can be used at less than or equal to 20 μg/mL.The antibody performs optimally with low pH antigen retrieval but high pH antigen retrieval can also be used.eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.
|Cutaneous Lymphocyte Antigen|
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
|Flow CytometryþFunctional AssayþImmunohistochemistryþWestern Blot|
For Research Use Only.