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Cutaneous Lymphocyte Antigen (CLA), eFluor 660, clone: HECA-452, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 50-9857-82

Code : NEW

Additional Details : Weight : 0.09500kg

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Product Code. 15500157

  • £144.97 / 100µg


EU Stock 2
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Description

Description

Description: This HECA-452 monoclonal antibody reacts with human Cutaneous Lymphocyte Antigen (CLA). This cell surface glycoprotein is most highly expressed on T cells in the skin. Nevertheless, CLA can also be detected on some peripheral T and NK cells, as well as monocytes, neutrophils and CD1a+ dendritic cells. Studies have determined that this antibody recognizes sialofucosylated glycans and sialyl Lewis x (sLex). CLA, which binds E-selectin and endothelial cell-leukocyte adhesion molecule 1 (ELAM-1), is involved in T lymphocyte infiltration and adhesion at cutaneous sites of inflammation.The HECA-452 antibody has been reported to inhibit sLex binding to E-selectin; however, this property is still under debate in the literature.Applications Reported: This HECA-452 antibody has been reported for use in flow cytometric analysis, western blotting, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections.Applications Tested: This HECA-452 antibody has been tested by flow cytometric analysis on normal human peripheral blood cells. This HECA-452 antibody has also been tested by immunhistochemistry on FFPE human tissue and can be used at less than or equal to 20 μg/mL.The antibody performs optimally with low pH antigen retrieval but high pH antigen retrieval can also be used.eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

Cutaneous Lymphocyte Antigen
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
CLA
IgM
100μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
Human
Flow CytometryþFunctional AssayþImmunohistochemistryþWestern Blot
HECA-452
eFluor 660
Conjugated
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
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