Thermo Scientific™ CloneJET™ PCR Cloning Kit PROMO

Efficiently clone PCR products generated with any thermostable DNA polymerase using Thermo Scientific™ CloneJET™ PCR Cloning Kit, a positive selection system.

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: K1231-RMP6225

20RXN CloneJET PCR Cloning Kit

UNSPSC: 41106617

Additional Details:
Additional Details: Weight: 0.00700kg



Product Code. 15513531

Quantity Price
1 £ 108.0 / 20 reactions
EU Stock 76
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Description and Specification

Highlights

The CloneJET PCR Cloning Kit contains a novel, ready-to-use positive selection cloning vector pJET1.2/blunt. The vector contains a lethal restriction enzyme gene that is disrupted by ligation of a DNA insert into the cloning site. As a result, only bacterial cells with recombinant plasmids are able to form colonies. Recircularized pJET1.2/blunt vector molecules lacking an insert express a lethal restriction enzyme, which kills the host E. coli cell after transformation. This positive selection drastically accelerates the process of colony screening and eliminates additional costs required for blue/white selection.

For convenience in mapping and manipulation of the insert, the pJET1.2/blunt cloning vector multiple cloning site contains two BglII recognition sequences that flank the insertion site. In addition, the vector contains a T7 promoter for in vitro and in vivo transcription as well as sequencing of the insert.

Highlights

  • Fast - PCR cloning in only 5 minutes
  • Highest efficiency - > 99% of positive clones
  • No cloning background - positive selection vector
  • Versatile - ideal for blunt-end or sticky-end cloning
  • Economical - no expensive blue/white screening