CD95 (APO-1/Fas), clone: 15A7, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-0951-85

Product Code. 15217127

Description

Description: The 15A7 monoclonal antibody reacts with mouse CD95 (Fas, Apo-1), a 40-50kDa member of the TNFR superfamily. CD95 is expressed by a broad range of hematopoietic and non-hematopoietic cells including monocytes, neutrophils, activated lymphocytes and fibroblasts. Interaction of CD95 on mature lymphocytes with its ligand (FasL) induces apoptosis and is thought to be important in peripheral tolerance. 15A7 reacts with mouse CD95 in BALB/c strain and a bit more weakly in C57Bl/6 strain. 15A7 does not induce apoptosis of Fas-expressing cells.Applications Reported: The 15A7 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 15A7 antibody has been tested by flow cytometric analysis of BALB/c splenocytes and thymocytes. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.

Specifications

• Antigen: CD95 (APO-1/Fas)
• Classification: Monoclonal
• Concentration: 0.5mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: P25446
• Gene Alias: ALPS1A, APO-1, APT1, Apo-1 Fas, CD95, FAS1, FASTM, TNFRSF6
• Host Species: Murine
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Type: Antibody
• Monoclonal or Polyclonal: Monoclonal
• Target Species: Murine

• Applications: Flow Cytometry
• Clone: 15A7
• Conjugate: Unconjugated
• Gene: Fas
• Format: Purified
• Gene Symbols: Fas
• Isotype: IgG1, kappa
• Quantity: 500μg
• Storage Requirements: Store at 2-8°C.
• Primary or Secondary: Primary
• Gene ID (Entrez): 14102

For Research Use Only.