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CD95 (APO-1/Fas), PE-Cyanine7, clone: DX2, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 25-0959-42

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15558446

  • £189.82 / 100 tests


EU Stock 3
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Description

Description

Description: The DX2 monoclonal antibody reacts with human CD95 (Fas, Apo-1), a 40-50kDa member of the TNFR superfamily. CD95 is expressed by a broad range of hematopoietic and non-hematopoietic cells including monocytes, neutrophils, lymphocytes and fibroblasts. Interaction of CD95 on mature lymphocytes with its ligand (FasL) induces apoptosis and is thought to be important in peripheral tolerance. DX2 does not block binding of EOS9.1, another antibody specific for human CD95.The DX2 monoclonal is reported to recognize dog/canine CD95.Applications Reported: This DX2 antibody has been reported for use in flow cytometric analysis.Applications Tested: This DX2 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (1 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD95 (APO-1/Fas)
Monoclonal
5μl/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
P25445
ALPS1A, APO-1, APT1, Apo-1 Fas, CD95, FAS1, FASTM, TNFRSF6
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
CanineĆ¾Human
Flow Cytometry
DX2
PE-Cyanine7
FAS
Conjugated
FAS
IgG1, kappa
100 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material. This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling and experimental procedures.
Primary
355
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