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CD90.1 (Thy-1.1) Mouse anti-Mouse, Rat, Super Bright 600, Clone: HIS51, eBioscience™

Mouse Monoclonal Antibody

£122.00 - £282.00

Specifications

Classification Monoclonal
Monoclonal or Polyclonal Monoclonal
Primary or Secondary Primary
Antigen CD90.1 (Thy-1.1)
Conjugate Super Bright 600
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Products 2
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
15963524
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Invitrogen
63-0900-80
25 μg
£122.00
25µg
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Estimated Shipment: 02-02-2022
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15973524
View Documents Promotion Details
Invitrogen
63-0900-82
100 μg
£282.00
100µg
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Save Up to 
»
Estimated Shipment: 02-02-2022
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Description

Description

Description: The HIS51 monoclonal antibody reacts with rat CD90 and cross-reacts with mouse CD90.1, a GPI-linked membrane molecule. In the rat, CD90 is expressed by hematopoietic stem cells, immature B cells, thymocytes, recent thymic emigrants, neurons, inflammed endothelia and other cell types. In the CD90.1-expressing mouse strains, PL and AKR, CD90 is expressed by early hematopoietic cells in the bone marrow, thymocytes and mature T cells. Applications Reported: This HIS51 antibody has been reported for use in flow cytometric analysis. Applications Tested: This HIS51 antibody has been tested by flow cytometric analysis of rat splenocytes. This may be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Super Bright 600 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 600 nm. We recommend using a 610/20 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome. When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Staining Buffer (Product No. SB-4400) to minimize any non-specific polymer interactions.

Please refer to the datasheet for Super Bright Staining Buffer for more information. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (Product No. 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product No. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 405 nm; Emission: 600 nm; Laser: Violet Laser CD90 (Thy 1) antigen is a GPI linked glycoprotein member of the Immunoglobulin super family. It is expressed in murine T cells, thymocytes, neural cells, Kupffer′s cells and fibroblasts. Thy 1 may play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.
Specifications

Specifications

Monoclonal
Primary
Super Bright 600
Monoclonal
CD90.1 (Thy-1.1)
SDS
Product Certifications