CD8b Monoclonal Antibody (eBioH35-17.2 (H35-17.2)), PE-Cyanine7, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Product Code	Brand	Quantity
15340890	Affymetrix eBioscience 25-0083-82	100 μg
15330890	Affymetrix eBioscience 25-0083-80	25 μg

Description

Description: The eBioH35-17.2 monoclonal antibody reacts with the mouse CD8 beta molecule. The CD8 beta chain associates with the CD8 alpha chain to form the CD8 alpha/beta heterodimer expressed on the surface of a majority of thymocytes, and on peripheral cytotoxic alpha beta TCR T cells. CD8 binds to MHC class I and plays a role in T cell development and activation of mature T cells. Applications Reported: This H35-17.2 antibody has been reported for use in flow cytometric analysis. Applications Tested: This eBioH35-17.2 (H35-17.2) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation.

CD8b Monoclonal antibody specifically detects CD8b in Mouse samples. It is validated for Flow Cytometry.

Specifications

• Antigen: CD8b
• Concentration: 0.2 mg/mL
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Gene Accession No.: P10300
• Gene Symbols: Cd8b1
• Primary or Secondary: Primary
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Gene: Cd8b1

• Clone: eBioH35-17.2 (H35-17.2)
• Applications: Flow Cytometry
• Conjugate: PE-Cyanine7
• Host Species: Rat
• Target Species: Mouse
• Gene ID (Entrez): 12526
• Isotype: IgG2b κ
• Purification Method: Affinity chromatography
• Product Type: Antibody

For Research Use Only.