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CD8a, Functional Grade, clone: 53-6.7, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 16-0081-82

Code : Z2

Additional Details : Weight : 0.01000kg

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Product Code. 15248367

  • £74.85 / 100µg


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Disclaimers:

For Research Use Only.


Description

Description

Description: The 53-6.7 monoclonal antibody reacts with the mouse CD8a molecule. CD8a is an approximately 32-34kDa cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha beta) or as a homodimer (CD8 alpha alpha). A majority of thymocytes and a subpopulation of mature alpha beta TCR T cells express CD8 alpha beta while gamma delta TCR T cells, a subpopulation of intestinal intraepithelial lymphocytes (IELs) and dendritic cells express CD8 alpha alpha. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T cell development and activation of mature T cells.Applications Reported: This 53-6.7 antibody has been reported for use in flow cytometric analysis. It has also been reported in T cell activation, isolation and depletion of CD8+ cells, and blocking of ligand binding.Applications Tested: The 53-6.7 antibody has been tested by flow cytometric analysis of mouse thymocytes and splenocytes. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.Storage and handling: Use in a sterile environment.Filtration: 0.2μm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.
Specifications

Specifications

CD8a
53-6.7
aqueous buffer, no sodium azide
CD8 alpha, Ly-2, Ly-35, Ly-B, Lyt-2
IgG2a
RUO
Primary
Murine
Functional Assay
Unconjugated
Purified
Rat
100μg
Store at 2-8°C.
Monoclonal
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