CD8a Rat anti-Mouse, Biotin, Clone: 4SM15, eBioscience™

Rat Monoclonal Antibody

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 13-0808-82

100UG Anti-Mouse CD8a Biotin

Code: NEW

Additional Details:
Additional Details: Weight: 0.09500kg



Disclaimers: For Research Use Only.

Product Code. 15587316

Quantity Price
1 £ 278.6 / 100µg
Estimated Shipment date
from Supplier 24-01-2017
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Description and Specification

Specification

Antigen CD8a
Applications Flow Cytometry
Applications Immunohistochemistry (Formalin/Paraffin)
Applications Microscopy
Clone 4SM15
Concentration 0.5mg/mL
Conjugate Biotin
Format Conjugated
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Gene Alias CD8 alpha, Ly-2, Ly-35, Lyt-2
Host Species Rat
Isotype IgG2a
Quantity 100μg
Regulatory Status RUO
Species Reactivity Mouse
Storage Requirements Store at 2-8°C. Do not freeze.
Primary or Secondary Primary
Monoclonal or Polyclonal Monoclonal

The 4SM15 monoclonal antibody reacts with the mouse CD8a molecule. CD8a is an approximately 32-34kDa cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha beta) or as a homodimer (CD8 alpha alpha). A majority of thymocytes and a subpopulation of mature alpha beta TCR T cells express CD8 alpha beta while gamma delta TCR T cells, a subpopulation of intestinal intraepithelial lymphocytes (IELs) and dendritic cells express CD8 alpha alpha. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T cell development and activation of mature T cells.

Recent studies have shown the 4SM15 antibody recognizes and binds a non-CD8a epitope in neural tissue. The 4SM15 clone is not recommended for staining of mouse neural tissue. For additional information please contact our technical service (tech@eBioscience.com).

The 4SM15 monoclonal antibody is not recommended for immunohistochemistry of frozen mouse tissue. For detection of mouse CD8a using immunohistochemistry of frozen tissue please refer to alternate product Anti-Mouse CD8a Purified (cat. 14-0081).