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CD8a, Biotin, clone: 4SM15, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 13-0808-80

Code : NEW

Additional Details : Weight : 0.09500kg

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Product Code. 15577316

  • £76.86 / 25µg

EU Stock 3
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For Research Use Only.



Description: The 4SM15 monoclonal antibody reacts with the mouse CD8a molecule. CD8a is an approximately 32-34kDa cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha beta) or as a homodimer (CD8 alpha alpha). A majority of thymocytes and a subpopulation of mature alpha beta TCR T cells express CD8 alpha beta while gamma delta TCR T cells, a subpopulation of intestinal intraepithelial lymphocytes (IELs) and dendritic cells express CD8 alpha alpha. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T cell development and activation of mature T cells.Recent studies have shown the 4SM15 antibody recognizes and binds a non-CD8a epitope in neural tissue. The 4SM15 clone is not recommended for staining of mouse neural tissue. For additional information please contact our technical service (tech@eBioscience.com). For detection of mouse CD8a in FFPE mouse neural tissue please refer to alternate product Anti-Mouse CD8a Purified (cat. no 14-0195).The 4SM15 monoclonal antibody is not recommended for immunohistochemistry of frozen mouse tissue. For detection of mouse CD8a using immunohistochemistry of frozen tissue please refer to alternate product Anti-Mouse CD8a Purified (cat. no. 14-0081).Applications Reported: This 4SM15 antibody has been reported for use in flow cytometric analysis, microscopy, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections.Applications Tested: This 4SM15 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded mouse tissue using low or high pH antigen retrieval and can be used at less than or equal to 10 μg/mL. The 4SM15 antibody has been tested by flow cytometric analysis of mouse splenocytes and can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Filtration: 0.2μm post-manufacturing filtered.


Store at 2-8°C. Do not freeze.
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
CD8 alpha, Ly-2, Ly-35, Lyt-2