CD8a, PE, clone: RPA-T8, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 12-0088-41
Code : NEW
Additional Details : Weight : 0.23750kg
DescriptionDescription: The RPA-T8 monoclonal antibody reacts with the human CD8a molecule, an approximately 32-34kDa cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha/beta) or as a homodimer (CD8 alpha/alpha). A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T-cell development and activation of mature T cells.Applications Reported: The RPA-T8 antibody been reported for use in flow cytometric analysis.Applications Tested: This RPA-T8 antibody is offered in 2 formats: -μg size: has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.25μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. - test size: has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at test size: 5μL (0.125μg) per test.The pre-titrated test size contains BSA and/or gelatin for protein stabilization.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2|
|CD8, Leu-2, MAL, p32, CD8a|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
For Research Use Only.