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CD86 (B7-2), FITC, clone: GL1, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 11-0862-82

Code : Z2

Additional Details : Weight : 0.25000kg

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Product Code. 15298139

  • £97.22 / 100µg


EU Stock 3
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Description

Description

Description: The GL1 monoclonal antibody reacts with mouse CD86, an ∽80kDa surface receptor also known as B7-2. CD86 and CD80 are members of the B7 family of costimulatory molecules. CD86 is expressed at low level on B cells, macrophages, and dendritic cells and is upregulated on B cells through a variety of surface stimuli including the BCR complex, CD40 and some cytokine receptors. CD86 is also expressed by activated mouse T cells and thioglycolate-elicited peritoneal cells. In addition to CD80 (B7-1), CD86 is a counter-receptor for the T cell surface molecules CD28 and CD152 (CTLA-4). This interaction plays a critical role in T-B crosstalk, T cell costimulation, autoantibody production and Th2-mediated Ig production. The kinetics of upregulation of CD86 upon stimulation, supports its major contribution during the primary phase of an immune response.Applications Reported: The GL1 antibody has been reported for use in flow cytometric analysis.Applications Tested: This GL1 antibody has been tested by flow cytometric analysis of stimulated mouse splenocytes. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD86
Monoclonal
0.5mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P42082
B7-2, B7.2, B70, CD28LG2, LAB72, MGC34413, B-lymphocyte activation antigen B7-2, B-lymphocyte antigen B7-2, B7-2 antigen, BU63, CD28 antigen ligand 2
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry
GL1
FITC
Cd86
Conjugated
Cd86
IgG2a, kappa
100μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
12524
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