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CD86 (B7-2), Functional Grade, clone: PO3.1, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 16-0861-81

Code : Z2

Additional Details : Weight : 0.01000kg

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Product Code. 15248517

  • £62.63 / 50µg


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Description

Description

Description: The PO3.1 monoclonal antibody reacts with mouse CD86, an ∽80kDa surface receptor also known as B7-2. CD86 and CD80 are members of the B7 family of costimulatory molecules. CD86 is expressed at low level on B cells, macrophages, and dendritic cells and is upregulated on B cells through a variety of surface stimuli including the BCR complex, CD40 and some cytokine receptors. CD86 is also expressed by activated mouse T cells and thioglycolate-elicited peritoneal cells. In addition to CD80 (B7-1), CD86 is a counter-receptor for the T cell surface molecules CD28 and CD152 (CTLA-4). The interaction of CD86 with its ligands plays a critical role in T-B crosstalk, T cell costimulation, autoantibody production and Th2-mediated Ig production. The kinetics of upregulation of CD86 upon stimulation supports its major contribution during the primary phase of an immune response.Applications Reported: The PO3.1 antibody has been reported for use in flow cytometric analysis. It has also been reported in blocking of CD86 in functional studies.Applications Tested: The PO3.1 antibody has been tested by flow cytometric analysis of resting and activated mouse splenocytes and can be used at 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.Storage and handling: Use in a sterile environment.Filtration: 0.2μm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.
Specifications

Specifications

CD86 (B7-2)
Monoclonal
1mg/mL
PBS with no preservative; pH 7.2
P42082
B7-2, B7.2, B70, CD28LG2, LAB72, MGC34413, B-lymphocyte activation antigen B7-2, B-lymphocyte antigen B7-2, B7-2 antigen, BU63, CD28 antigen ligand 2
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Functional Assay
PO3.1
Functional Grade
Cd86
Purified
Cd86
IgG2b, kappa
50μg
Store at 2-8°C.
Primary
12524
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