missing translation for 'onlineSavingsMsg'
Learn More

CD86 (B7-2), Biotin, clone: IT2.2, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 13-0869-80

Code : Z2

Additional Details : Weight : 0.25000kg

 View more versions of this product

Product Code. 15269639

  • £56.38 / 25µg


Estimated Shipment date
from Supplier 30-05-2018
Add to basket

Description

Description

Description: The IT2.2 monoclonal antibody reacts with human CD86, an ∽80kDa surface receptor also known as B7-2. CD86 and CD80 are members of the B7 family of costimulatory molecules. CD86 is expressed at low levels on B cells, macrophages, and dendritic cells and is upregulated on B cells through a variety of surface stimuli including the BCR complex, CD40 and some cytokine receptors. In addition to CD80 (B7-1), CD86 is a counter-receptor for the T cell surface molecules CD28 and CD152 (CTLA-4). The interaction of CD86 with its ligands plays a critical role in T-B crosstalk, T cell costimulation, autoantibody production and Th2-mediated Ig production. The kinetics of upregulation of CD86 upon stimulation supports its major contribution during the primary phase of an immune response.Applications Reported: The IT2.2 antibody has been reported for use in flow cytometric analysis.Applications Tested: The IT2.2 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD86 (B7-2)
Monoclonal
0.5mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P42081
B7-2, B7.2, B70, CD28LG2, LAB72, MGC34413, B-lymphocyte activation antigen B7-2, B-lymphocyte antigen B7-2, B7-2 antigen, BU63, CD28 antigen ligand 2
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
IT2.2
Biotin
CD86
Conjugated
CD86
IgG2b, kappa
25μg
Store at 2-8°C. Do not freeze.
Primary
942
Videos

For Research Use Only.