CD85d (ILT4) Rat anti-Human, PE, Clone: 42D1, eBioscience™

Rat Monoclonal Antibody

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 12-5149-41

Code: NEW

Additional Details:
Additional Details: Weight: 0.23750kg



Disclaimers: For Research Use Only.

Product Code. 15576996

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Description and Specification

Specification

Monoclonal or Polyclonal Monoclonal
Clone 42D1
Applications Flow Cytometry
Storage Requirements Store at 2-8°C. Do not freeze. Light-sensitive material.
Regulatory Status RUO
Conjugate PE
Concentration 5μL (1μg)/test
Antigen CD85d (ILT4)
Isotype IgG2a
Quantity 25 tests
Format Conjugated
Species Reactivity Human
Primary or Secondary Primary
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Gene Alias LIR2, LILRB2, MIR10
Host Species Rat

The monoclonal antibody 42D1 recognizes the CD85d protein also known as ILT4, LIR-2. It is a member of the ILT (immunoglobulin-like transcript)/ LIR (leukocyte Ig-like receptor), MIR (monocytes Ig-like receptor) family. CD85d is a 110kDa single transmembrane receptor with 3 Ig-like domains and a long cytoplasmic domain containing ITIMs (immunoreceptor tyrosine-based inhibitory motifs). Expression is confined to monocytes, macrophages and dendritic cells. The ligand for CD85d is non-classical class I HLA-G, -B and G1. Upon ligand binding, tyrosine phosphatase SHP-1 interacts with the ITIM. Interaction of HLA-G with ILT4/CD85d is thought to contribute to tolerance during fetal implantation. Additionally, it is the levels of ILT3 and ILT4 on DCs that suggests a role in organ allograft rejection.

Crosslinking of the receptor with the monoclonal antibody 42D1 inhibits calcium flux as well as enhancing HLA-G tetramer staining and restoring the stimulating activity of tolerogenic DC.