CD85d (ILT4) Monoclonal Antibody (42D1), APC, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 17-5149-42

Product Code. 15548116

Description

Description: The monoclonal antibody 42D1 recognizes the CD85d protein also known as ILT4, LIR-2. It is a member of the ILT (immunoglobulin-like transcript)/ LIR (leukocyte Ig-like receptor), MIR (monocytes Ig-like receptor) family. CD85d is a 110 kDa single transmembrane receptor with 3 Ig-like domains and a long cytoplasmic domain containing ITIMs (immunoreceptor tyrosine-based inhibitory motifs). Expression is confined to monocytes, macrophages and dendritic cells. The ligand for CD85d is non-classical class I HLA-G, -B and G1. Upon ligand binding, tyrosine phosphatase SHP-1 interacts with the ITIM. Interaction of HLA-G with ILT4/CD85d is thought to contribute to tolerance during fetal implantation. Additionally, it is the levels of ILT3 and ILT4 on DCs that suggests a role in organ allograft rejection. Crosslinking of the receptor with the monoclonal antibody 42D1 inhibits calcium flux as well as enhancing HLA-G tetramer staining and restoring the stimulating activity of tolerogenic DC. Applications Reported: This 42D1 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 42D1 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL.

CD85d (ILT4) Monoclonal antibody specifically detects CD85d (ILT4) in Human samples. It is validated for Flow Cytometry.

Specifications

• Antigen: CD85d (ILT4)
• Classification: Monoclonal
• Concentration: 5 μL/Test
• Formulation: PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
• Gene Accession No.: Q8N423
• Gene Symbols: LILRB2
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 10288
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Form: Liquid

• Applications: Flow Cytometry
• Clone: 42D1
• Conjugate: APC
• Gene: LILRB2
• Gene Alias: CD85 antigen-like family member D; CD85D; CD85d antigen; Ig-like transcript 4; ILT4; ILT-4; leucocyte Ig-like receptor B2; leukocyte immunoglobulin like receptor B2; leukocyte immunoglobulin-like receptor subfamily B member 2; leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 2; leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 3; LILRB2; Lilrb3; LIR2; LIR-2; LIR2CD85D; MIR10; MIR-10; MIR10LILRA6; monocyte/macrophage immunoglobulin-like receptor 10; myeloid inhibitory receptor 10; Neutrophil immunoglobulin-like receptor 1; Nilr1; NILR-1; paired-Ig-like receptor B; Pirb; type 1 one-pass transmembrane Ig-like receptor
• Host Species: Rat
• Quantity: 100 Tests
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG2a κ

For Research Use Only.