missing translation for 'onlineSavingsMsg'
Learn More

CD83 Monoclonal Antibody (HB15e), PE-Cyanine7, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 25-0839-41

Code : NEW

Additional Details : Weight : 0.23750kg

 View more versions of this product

Product Code. 15568436

This item is currently unavailable or has been discontinued.
View the product page for possible alternatives.
View alternative products »


Description

Description

Description: The HB15e monoclonal antibody reacts with human CD83, a 45 kDa transmembrane glycoprotein. CD83, a member of the Ig superfamily, is expressed on cultured dendritic cells, interdigitating, follicular, and circulating dendritic cells as well as some proliferating lymphocytes, and human cell lines express this antigen. While the function of CD83 is unclear, it can serve as a useful marker for mature human blood dendritic cells. Applications Reported: This HB15e antibody has been reported for use in flow cytometric analysis. Applications Tested: This HB15e antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation.

Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 μm post-manufacturing filtered. CD83 cell surface antigen is a 40-45kD glycoprotein expressed by peripheral blood dendritic cells. Peripheral lymphocytes can be induced to express very low levels of CD83 after culture in agents such as Con A or PHA. In immunohistology, CD83 is shown to be expressed strongly by interfollicular interdigitating reticulum cells and more weakly by cells within germinal centres. CD83 is also expressed by Langerhan′s cells in the skin. The CD83 antigen is a 186-amino-acid single-chain glycoprotein and this molecule is a member of the immunoglobulin superfamily that is composed of an extracellular V-type Ig-like single domain, a transmembrane region, and a short, 40-amino-acid cytoplasmic tail. CD83 antigen undergoes extensive post-translational glycosylation, since the determined Mr is twice the predicted size of the core protein. However, CD83+ cells have a unique cell surface immuno-phenotype that does not correlate with that of T cells, B cells, NK cells, or cells of the myelomonocytic lineage. CD83+ cells coexpress the highest levels of MHC class II molecules, when compared with other leucocyte lineages. They also co-express T cell markers (CD2, CD5), B cell markers (CD40, CD78), myeloid cell markers (CD13, CD33, CD36), cytokine receptors as well as other cell surface molecules. Diseases associated with CD83 dysfunction include plague and Rift Valley Fever.
Specifications

Specifications

CD83
Monoclonal
5 μL/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
Q01151
Mouse
25 Tests
Primary
Human
Antibody
IgG1 κ
Flow Cytometry
HB15e
PE-Cyanine7
CD83
CD83
Affinity chromatography
RUO
9308
4° C, store in dark, DO NOT FREEZE!
Liquid
Product Suggestions

Product Suggestions

Videos
SDS
Documents

Documents

Certificates
Special Offers

Special Offers

For Research Use Only.