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CD80 (B7-1), PE, clone: 3H5, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 12-0800-82

Code : Z2

Additional Details : Weight : 0.25000kg

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Product Code. 15298589

  • £112.38 / 100µg


Estimated Shipment date
from Supplier 04-06-2018
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Description

Description

Description: The 3H5 monoclonal antibody reacts with rat CD80 (B7-1), an ∽70kDa member of the Ig superfamily. CD80 is expressed by macrophages, dendritic cells and activated B cells. CD80 has high affinity for binding to two T cell surface antigens, CD28 and CD152 (CTLA-4). The interaction of CD28 and CD152 with CD80 is crucial in T-B cell communication leading to activation of T and B cells, respectively. 3H5 blocks binding of CD80 to its ligands.Applications Reported: The 3H5 antibody has been reported for use in flow cytometric analysis.Applications Tested: The 3H5 antibody has been tested by flow cytometric analysis of activated rat splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD80
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
Conjugated
Cd80
IgG1, kappa
100μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
25408
Flow Cytometry
3H5
PE
Cd80
B7-1, B7.1, Cd28l, Ly-53, Ly53, MIC17, TS/A-1, LAB7, B7.1, CD28LG, CD28LG1
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Rat
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