CD80 (B7-1), PE, clone: 3H5, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 12-0800-83
Code : Z2
Additional Details : Weight : 0.25000kg
DescriptionDescription: The 3H5 monoclonal antibody reacts with rat CD80 (B7-1), an ∽70kDa member of the Ig superfamily. CD80 is expressed by macrophages, dendritic cells and activated B cells. CD80 has high affinity for binding to two T cell surface antigens, CD28 and CD152 (CTLA-4). The interaction of CD28 and CD152 with CD80 is crucial in T-B cell communication leading to activation of T and B cells, respectively. 3H5 blocks binding of CD80 to its ligands.Applications Reported: The 3H5 antibody has been reported for use in flow cytometric analysis.Applications Tested: The 3H5 antibody has been tested by flow cytometric analysis of activated rat splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
|aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|