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CD80 (B7-1), PerCP-eFluor™ 710, clone: 2D10.4, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 46-0809-42

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15519156

  • £234.55 / 100 tests


EU Stock 12
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Disclaimers:

For Research Use Only.


Description

Description

Description: The 2D10.4 monoclonal antibody reacts with human CD80 (B7-1), a 60kDa member of the Ig superfamily. CD80 is expressed by activated B cells, macrophages and dendritic cells. In addition, activated T cells express this antigen. CD80 has high affinity for binding to two T cell surface antigens, CD28 and CD152 (CTLA-4). The interaction of CD28 and CD152 with CD80 is crucial in T-B cell communication leading to activation of T and B cells, respectively.Applications Reported: This 2D10.4 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 2D10.4 antibody has been pre-titrated and tested by flow cytometric analysis of Daudi cell line. This can be used at 5 μL (0.5 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD80
2D10.4
PerCP-eFluor 710
Conjugated
Murine
100 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
Monoclonal
Flow Cytometry
5μL (0.5μg)/test
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
B71, BB1
IgG1
RUO
Primary
Human
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