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CD80 (B7-1), PE-Cyanine5, clone: 16-10A1, eBioscience™

Armenian Hamster Monoclonal Antibody

Brand:  Affymetrix eBioscience 15-0801-82

Code : Z2

Additional Details : Weight : 0.25000kg

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Product Code. 15390490

  • £171.27 / 100µg


EU Stock 2
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Description

Description

Description: The 16-10A1 monoclonal antibody reacts with mouse CD80 (B7-1), a 55kDa member of the Ig superfamily. CD80 is expressed by macrophages, dendritic cells and activated B cells. In addition, activated T cells express this antigen. CD80 has high affinity for binding to two T cell surface antigens, CD28 and CD152 (CTLA-4). The interaction of CD28 and CD152 with CD80 is crucial in T-B cell communication leading to activation of T and B cells, respectively.Applications Reported: The 16-10A1 antibody has been reported for use in flow cytometric analysis.Applications Tested: The 16-10A1 antibody has been tested by flow cytometric analysis of activated mouse splenocytes. This can be used at less than or equal to 0.06 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD80
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
Q00609
B7-1, B7.1, Cd28l, Ly-53, Ly53, MIC17, TS/A-1, LAB7, B7.1, CD28LG, CD28LG1
Armenian Hamster
Affinity chromatography
RUO
Antibody
Monoclonal
Porcine
Flow Cytometry
16-10A1
PE-Cyanine5
Cd80
Conjugated
Cd80
IgG
100μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
12519
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