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CD80 (B7-1), eFluor 450, clone: 16-10A1, eBioscience™

Armenian Hamster Monoclonal Antibody

Brand:  Affymetrix eBioscience 48-0801-80

Code : NEW

Additional Details : Weight : 0.09500kg

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Product Code. 15549696

  • £81.11 / 25µg


Estimated Shipment date
from Supplier 28-03-2018
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Disclaimers:

For Research Use Only.


Description

Description

Description: The 16-10A1 monoclonal antibody reacts with mouse CD80 (B7-1), a 55kDa member of the Ig superfamily. CD80 is expressed by macrophages, dendritic cells and activated B cells. In addition, activated T cells express this antigen. CD80 has high affinity for binding to two T cell surface antigens, CD28 and CD152 (CTLA-4). The interaction of CD28 and CD152 with CD80 is crucial in T-B cell communication leading to activation of T and B cells, respectively.Applications Reported: This 16-10A1 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 16-10A1 antibody has been tested by flow cytometric analysis of stimulated mouse splenocytes. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD80 (B7-1)
16-10A1
eFluor 450
Conjugated
Armenian Hamster
25μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Monoclonal
Flow Cytometry
0.2mg/mL
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
B71, Ly-53
IgG
RUO
Primary
Porcine
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