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CD80 (B7-1), clone: 16-10A1, eBioscience™

Armenian Hamster Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-0801-81

Code : Z2

Additional Details : Weight : 0.01000kg

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Product Code. 15247077

  • £48.31 / 50µg


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For Research Use Only.


Description

Description

Description: The 16-10A1 monoclonal antibody reacts with mouse CD80 (B7-1), a 55kDa member of the Ig superfamily. CD80 is expressed by macrophages, dendritic cells and activated B cells. In addition, activated T cells express this antigen. CD80 has high affinity for binding to two T cell surface antigens, CD28 and CD152 (CTLA-4). The interaction of CD28 and CD152 with CD80 is crucial in T-B cell communication leading to activation of T and B cells, respectively.Applications Reported: This 16-10A1 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistology staining of frozen tissue sections. 16-10A1 has also been reported in blocking of CD80 to its ligands. (Please use Functional Grade purified 16-10A1, cat. 16-0801, in functional assays.).Applications Tested: The 16-10A1 antibody has been tested by flow cytometric analysis of activated mouse splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD80 (B7-1)
16-10A1
Unconjugated
Purified
Armenian Hamster
50μg
Store at 2-8°C.
Monoclonal
Immunoprecipitation
0.5mg/mL
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
B71, Ly-53
IgG
RUO
Primary
Porcine
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