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CD79a, eFluor 660, clone: 24C2.5, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 50-0791-82

Code : Z2

Additional Details : Weight : 0.25000kg

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Product Code. 15381870

  • £189.15 / 100µg


Estimated Shipment date
from Supplier 24-07-2018
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Description

Description

Description: This 24C2.5 monoclonal antibody reacts with the intracellular tail of mouse CD79a (also known as Ig alpha or mb-1), a signaling component of the B cell receptor (BCR). CD79a heterodimerizes with CD79b (Ig beta, B29), and together with surface Ig, make up the BCR. Both CD79 subunits consist of a single extracellular Ig domain, a transmembrane domain, and an intracellular signaling domain. CD79a is expressed almost exclusively on B cells, as well as in most B lineage acute lymphoblastic leukemias. The cytoplasmic domain of CD79a contains immunoreceptor tyrosine-based activation motifs (ITAMs), which constitute the signal transducing portions of the BCR. Tyrosine phosphorylation of these ITAMs by Syk and Lyn initiates numerous signaling cascades, resulting in B cell activation, proliferation, and differentiation.Applications Reported: This 24C2.5 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This 24C2.5 antibody has been tested by intracellular staining and flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD79a
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P11911
IGA, MB-1
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry
24C2.5
eFluor 660
Cd79a
Conjugated
Cd79a
IgG1, kappa
100μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
12518
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