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CD75, eFluor 660, clone: LN-1, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 50-0759-42

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15529896

  • £157.73 / 100 tests


Estimated Shipment date
from Supplier 25-05-2018
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Description

Description

Description: This LN-1 monoclonal antibody reacts with human CD75, a cell surface carbohydrate determinant produced by alpha-2,6-sialytransferase. CD75 is expressed predominantly on mature B cells in peripheral blood and germinal centers, as well as several B cell lines (e.g., Raji, Ramos). CD75 is also expressed on non-immune tissues such as breast, prostate, colon, liver, and kidney. Reports demonstrate that CD75 is a ligand of CD22.Studies have shown that, depending on fixation and tissue processing, the LN-1 antibody also recognizes astrocytes and microglia in the human brain.Applications Reported: This LN-1 antibody has been reported for use in flow cytometric analysis.Applications Tested: This LN-1 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD75
Monoclonal
5μl/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
P15907
Sialyltransferase 1, SIAT1, ST6GalI, ST6N, B-cell antigen CD75, CMP-N-acetylneuraminate beta-galactosamide alpha-2, 6-sialyltransferase, CMP-N-acetylneuraminate-beta-galactosamide-alpha-2, 6-sialyltransferase 1, ST6Gal I, alpha 2, 6-ST 1, beta-galactoside alpha-2, 6-sialyltransferase 1, sialyltransferase 1
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
LN-1
eFluor 660
ST6GAL1
Conjugated
ST6GAL1
IgM
100 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
6480
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