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CD70, Functional Grade, clone: FR70, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 16-0701-85

Code : Z2

Additional Details : Weight : 0.01000kg

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Product Code. 15298497

  • £189.15 / 500µg


Estimated Shipment
28-02-2018
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Disclaimers:

For Research Use Only.


Description

Description

Description: The FR70 monoclonal antibody reacts with mouse CD70, a 30-33kDa type II transmembrane glycoprotein. CD70 is a member of the TNF family and is expressed by activated mouse T and B lymphocytes. Interaction of CD70 with CD27, its ligand on T cells, is important in T-B cell crosstalk and plays a role in costimulation and immune regulation.Applications Reported: The FR70 antibody has been reported for use in flow cytometric analysis. FR70 has also been reported in blocking of CD70 in vitro .Applications Tested: The FR70 antibody has been tested by flow cytometric analysis of LPS-stimulated mouse splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.Storage and handling: Use in a sterile environment.Filtration: 0.2μm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.
Specifications

Specifications

CD70
FR70
Unconjugated
Purified
Rat
500μg
Store at 2-8°C.
Monoclonal
Functional Assay
1mg/mL
aqueous buffer, no sodium azide
TNFSF7, Ki-24, CD27 ligand
IgG2b
RUO
Primary
Murine
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