CD70, clone: FR70, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 14-0701-85
Code : Z2
Additional Details : Weight : 0.01000kg
DescriptionDescription: The FR70 monoclonal antibody reacts with mouse CD70, a 30-33kDa type II transmembrane glycoprotein. CD70 is a member of the TNF family and is expressed by activated mouse T and B lymphocytes. Interaction of CD70 with CD27, its ligand on T cells, is important in T-B cell crosstalk and plays a role in costimulation and immune regulation.Applications Reported: The FR70 antibody has been reported for use in flow cytometric analysis, and immunoprecipitation. FR70 has also been reported in blocking of CD70 in vitro . (Please use Functional Grade purified FR70, cat. 16-0701, in functional assays.).Applications Tested: The FR70 antibody has been tested by flow cytometric analysis of LPS-stimulated mouse splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.09% sodium azide; pH 7.2|
|CD27LG, Cd27l, Tnfsf7|
|Flow CytometryþFunctional AssayþImmunoprecipitation|
|Store at 2-8°C.|
For Research Use Only.