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CD69, PE-Cyanine7, clone: FN50, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 25-0699-42

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15598426

  • £182.82 / 100 tests


EU Stock 8
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Description

Description

Description: The FN50 monoclonal antibody reacts with human CD69, also known as very early activation antigen (VEA). CD69 is approximately 30kDa and is expressed on the cell-surface as a disulfide-linked dimer. CD69 is rapidly upregulated upon activation and expressed on lymphocytes, monocytes and platelets.Applications Reported: This FN50 antibody has been reported for use in flow cytometric analysis.Applications Tested: This FN50 antibody has been pre-titrated and tested by flow cytometric analysis of resting and 6-hour TPA-activated human normal human peripheral blood cells. This can be used at 5 μL (0.06 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD69
Monoclonal
5μl/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
Q07108
AIM, BL-AC/P26, CLEC2C, EA1, GP32/28, MLR-3, C-type lectin domain family 2, member C, CD69 antigen
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
FN50
PE-Cyanine7
CD69
Conjugated
CD69
IgG1, kappa
100 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material. This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling and experimental procedures.
Primary
969
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