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CD69, eFluor 450, clone: H1.2F3, eBioscience™

Armenian Hamster Monoclonal Antibody

Brand:  Affymetrix eBioscience 48-0691-80

Code : Z2

Additional Details : Weight : 0.25000kg

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Product Code. 15371730

  • £62.64 / 25µg


EU Stock 6
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Description

Description

Description: The H1.2F3 monoclonal antibody reacts with mouse CD69, also known as very early activation antigen (VEA). CD69 is approximately 35kDa and is expressed on the surface as a disulfide-linked dimer. While a small subset of lymphocytes in the thymus, spleen and lymph nodes express this antigen, activation of both T and B cells rapidly upregulates the surface expression of CD69, suggesting a role for CD69 in lymphocyte development and activation.Applications Reported: This H1.2F3 antibody has been reported for use in flow cytometric analysis.Applications Tested: This H1.2F3 antibody has been tested by flow cytometric analysis of 24 hour Con A-stimulated mouse splenocytes. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD69
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P37217
AIM, BL-AC/P26, CLEC2C, EA1, GP32/28, MLR-3, C-type lectin domain family 2, member C, CD69 antigen
Armenian Hamster
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry
H1.2F3
eFluor 450
Cd69
Conjugated
Cd69
IgG
25μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
12515
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