CD68, eFluor 570, clone: 815CU17, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 41-0687-80

Product Code. 15518926

Description

CD68 (Macrosialin) is a 110kDa integral membrane glycoprotein predominantly expressed on the intracellular lysomsomes of monocytes and macrophages and to a lesser extent by dendritic cells and peripheral blood granulocytes. Elevated expression of CD68 has been demonstrated on CD34+ cells in various human malignancies, including several Acute Myeloid Leukemia studies.

Description: This 815CU17 monoclonal antibody reacts with human CD68, a 110 kDa, transmembrane glycoprotein that belongs to the sialomucin family and is closely related to the lysosomal-associated membrane proteins (LAMP) and scavenger receptor. CD68 is predominantly an intracellular protein, found mainly in the late endosomal compartment but can be detected in smaller amounts on the surface of mainly myeloid-derived cells: monocytes, macrophages, dendritic cells (and in some langerhans cells), neutrophils, basophils, mast cells, myeloid progenitor cells, and a subset of CD34+ hematopoietic bone marrow progenitor cells. The function has not been fully elucidated but based on homology and structure, CD68 may play a role in antigen processing or presentation and protection of the lysosomal membrane from hydrolytic enzymes. Reports have also shown expression in activated T cells, and about 40% of peripheral blood B lymphocytes and 50% of all B-ALL.This 815CU17 antibody does not cross-react with mouse or rat CD68.For flow cytometric analysis of human CD68, we recommend clone eBioY1/82A.Applications Reported: This 815CU17 antibody has been reported for use in microscopy and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections.Applications Tested: This 815CU17 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded human tissue using low or high pH antigen retreival and can be used at less than or equal to 10 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Filter Recommendation: When using this eFluor™ 570 antibody conjugate, we recommend a filter that will capture the 570 emission wavelength (for example, Excitation 545/25, 565LP, Emission 605/70). A standard Alexa Fluor™ 555 or TRITC filter is acceptable.Excitation: 555 nm; Emission: 570 nm

Specifications

• Antigen: CD68
• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Formulation: PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
• Gene Accession No.: P34810
• Host Species: Mouse
• Quantity: 25 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG1 κ

• Applications: Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
• Clone: 815CU17
• Conjugate: eFluor 570
• Gene: CD68
• Gene Symbols: CD68
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 968
• Content And Storage: Store at 2-8°C. Do not freeze. Light-sensitive material.
• Form: Liquid

For Research Use Only.