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CD65, FITC, clone: VIM8, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 11-0659-41

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15556436

  • £74.36 / 25 tests


EU Stock 2
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Description

Description

Description: This VIM8 antibody reacts with the desialylated asialoganglioside of human CD65. This type 2 fucosylated carbohydrate antigen is expressed on the surface of all myeloid cells during development. Moreover, CD65 is expressed highly on granulocytes and weakly on monocytes in peripheral blood. CD65 has been reported to be a ligand for CD62E (E-Selectin). However, the sialylated form of CD65 (designated CD65s) can be used as a marker for acute myeloid leukemia (AML) as its expression increases as CD34 decreases. Finally, CD65 is important for extravascular infiltration of AML cells.Applications Reported: This VIM8 antibody has been reported for use in flow cytometric analysis.Applications Tested: This VIM8 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD65
Monoclonal
5μl/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
Fucoganglioside Type II, Fucoganglioside Type 2
IgM
25 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
Human
Flow Cytometry
VIM8
FITC
Conjugated
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
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