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CD63, eFluor 450, clone: H5C6, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 48-0639-41

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15589686

  • £82.61 / 25 tests


Estimated Shipment date
from Supplier 28-02-2018
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Disclaimers:

For Research Use Only.


Description

Description

Description: This H5C6 monoclonal antibody reacts with human CD63, a type III member of the tetraspanin family of transmembrane proteins. CD63 is expressed intracellularly on lysosomes, endosomes, and granules of resting platelets and basophils. However, cell surface expression of CD63 can be detected on activated basophils and platelets, monocytes, macrophages, and granulocytes. This receptor is also expressed on endothelial cells, fibroblasts, and smooth muscle cells. Studies have demonstrated that CD63 associates with integrins (VLA-3 and VLA-6) and TIMP-1 to mediate the allergic response.Applications Reported: This H5C6 antibody has been reported for use in flow cytometric analysis.Applications Tested: This H5C6 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (1 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD63
H5C6
eFluor 450
Conjugated
Murine
25 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
Monoclonal
Flow Cytometry
5μL (1.0μg)/test
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Lysosome-associated membrane protein 3, Lamp 3
IgG1
RUO
Primary
Human
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