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CD62P (P-Selectin), Functional Grade, clone: Psel.KO2.12, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 16-0622-81

Code : Z2

Additional Details : Weight : 0.01000kg

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Product Code. 15298487

  • £74.60 / 50µg


EU Stock 1
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Disclaimers:

For Research Use Only.


Description

Description

Description: CD62P (also known as P-selectin and GMP-140) is a 140-kDa cell adhesion molecule that can be induced on endothelial cells and platelets. During inflammation, this selectin mediates adhesion of monocytes and neutrophils to activated platelets, as well as leukocyte rolling on endothelial cells. The major ligand for CD62P on neutrophils is P-selectin glycoprotein ligand-1 (PSGL-1). This monoclonal antibody recognizes an epitope that is conserved across several species (e.g. mouse, human, and rat).Applications Reported: This Psel.KO2.12 antibody has been reported for use in flow cytometric analysis and for blocking CD62P-mediated adhesion.Applications Tested: This Psel.KO2.12 antibody has been tested by flow cytometric analysis on thrombin-activated mouse platelets. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.Storage and handling: Use in a sterile environment.Filtration: 0.2μm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.
Specifications

Specifications

CD62P (P-Selectin)
Psel.KO2.12
Unconjugated
Purified
Murine
50μg
Store at 2-8°C.
Monoclonal
Functional Assay
1mg/mL
aqueous buffer, no sodium azide
LECAM-3
IgG1
RUO
Primary
Murine
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