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CD62L (L-Selectin), eVolve 605, clone: MEL-14, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 83-0621-41

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15580757

This item has been discontinued and is no longer available. View the product for possible alternatives or contact our Product Support team on 01509 555888 for assistance


Description

Description

Description: The MEL-14 monoclonal antibody reacts with mouse CD62L, a 76kDa member of the selectin family. CD62L is expressed by neutrophils, monocytes, and subsets of T, B, and NK cells and binds a number of glycosylated, fucosylated, sulfated sialylated glycoproteins including CD34, glycam-1 and MAdCam-1. These interactions mediate rolling of lymphocytes on activated endothelium at the sites of inflammation and homing of cells to the high endothelial venules (HEV) of peripheral lymphoid tissues.Applications Reported: This MEL-14 antibody has been reported for use in flow cytometric analysis.Applications Tested: This MEL-14 antibody has been pre-titrated and tested by flow cytometric analysis of mouse splenocytes. This can be used at 5 μL per test. A test is defined as the amount of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. For best results when using antibodies conjugated to eVolve™, we recommend a quick spin of the vial before use and avoid touching the bottom of the vial with the pipet tip.Laser/Filter Recommendation: eVolve™ 605 can be optimally excited with laser lines from 325-405 nm, and emits near 610 nm. We recommend using the violet (405 nm) laser line for excitation and a 595LP with a 605/40 or 610/20 bandpass filter, or equivalent, for detection.Fixation Recommendation: Antibodies conjugated to eVolve™ are compatible with eBioscience's fixation and permeabilization buffers.Excitation: 350-590 nm; Emission: 605 nm; Laser: Ultraviolet Laser, Violet LaserStorage and handling: For best results when using antibodies conjugated to eVolve™, we recommend a quick spin of the vial before use and avoid touching the bottom of the vial with the pipet tip.
Specifications

Specifications

CD62L (L-selectin)
Monoclonal
5μl/Test
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
P18337
SELL, LAM-1, LAM1, LECAM1, LNHR, LSEL, LYAM1, Leu-8, Lyam-1, PLNHR, TQ1, hLHRc
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry
MEL-14
eVolve 605
Sell
Conjugated
Sell
IgG2a, kappa
25 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material. This product should be used within 6 months from the date of receipt.
Primary
20343
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