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CD62L (L-Selectin), Biotin, clone: MEL-14, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 13-0621-85

Code : Z2

Additional Details : Weight : 0.25000kg

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Product Code. 15289609

  • £155.76 / 500µg


Estimated Shipment date
from Supplier 04-04-2018
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Description

Description

Description: The MEL-14 monoclonal antibody reacts with mouse CD62L, a 76kDa member of the selectin family. CD62L is expressed by neutrophils, monocytes, and subsets of T, B, and NK cells and binds a number of glycosylated, fucosylated, sulfated sialylated glycoproteins including CD34, glycam-1 and MAdCam-1. These interactions mediate rolling of lymphocytes on activated endothelium at the sites of inflammation and homing of cells to the high endothelial venules (HEV) of peripheral lymphoid tissues.Applications Reported: The MEL-14 antibody has been reported for use in flow cytometric analysis.Applications Tested: The MEL-14 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD62L (L-selectin)
MEL-14
Biotin
Conjugated
Rat
500μg
Store at 2-8°C. Do not freeze.
Monoclonal
Flow Cytometry
0.5mg/mL
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
LECAM-1, Ly-22
IgG2a, kappa
RUO
Primary
Murine
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