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CD62L (L-Selectin), Alexa Fluor 700, clone: MEL-14, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 56-0621-80

Code : Z2

Additional Details : Weight : 0.25000kg

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Product Code. 15342130

  • £84.99 / 25µg


Estimated Shipment date
from Supplier 02-07-2018
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Description

Description

Description: The MEL-14 monoclonal antibody reacts with mouse CD62L, a 76kDa member of the selectin family. CD62L is expressed by neutrophils, monocytes, and subsets of T, B, and NK cells and binds a number of glycosylated, fucosylated, sulfated sialylated glycoproteins including CD34, glycam-1 and MAdCam-1. These interactions mediate rolling of lymphocytes on activated endothelium at the sites of inflammation and homing of cells to the high endothelial venules (HEV) of peripheral lymphoid tissues.Applications Reported: This MEL-14 antibody has been reported for use in flow cytometric analysis.Applications Tested: This MEL-14 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.06 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Alexa Fluor® 700 emits at 723 nm and can be excited with the red laser (633 nm). Most instruments will require a 685 LP mirror and 710/20 filter. Please make sure that your instrument is capable of detecting this fluorochrome.Excitation: 633-647 nm; Emission: 723 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD62L (L-selectin)
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P18337
SELL, LAM-1, LAM1, LECAM1, LNHR, LSEL, LYAM1, Leu-8, Lyam-1, PLNHR, TQ1, hLHRc
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry
MEL-14
Alexa Fluor 700
Sell
Conjugated
Sell
IgG2a, kappa
25μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
20343
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