CD62E (E-selectin), clone: P2H3, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 14-0627-80
Code : Z2
Additional Details : Weight : 0.01000kg
DescriptionDescription: The P2H3 monoclonal antibody reacts with human CD62E, a 97-115kDa member of the selectin family. CD62E, also known as E-selectin or endothelial-leukocyte adhesion molecule-1 (ELAM-1) is an adhesion molecule expressed by endothelial cells upon stimulation with cytokines including TNFalpha and IL-1beta. Induced expression of CD62E during inflammatory conditions is thought to mediate leukocyte rolling including the initial interaction of neutrophils with endothelium.The P2H3 monoclonal antibody also inhibits cellular adhesion to cytokine-activated endothelial cells.Applications Reported: This P2H3 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining.Applications Tested: This P2H3 antibody has been tested by flow cytometric analysis of TNF alpha-actiavted Human Umbilical Vein Endothelial Cells (HUVEC). This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.09% sodium azide; pH 7.2|
|RP1-117P20.2, CD62E, ELAM, ELAM1, ESEL, LECAM2|
|Flow CytometryþFunctional AssayþImmunohistochemistryþImmunoprecipitation|
|Store at 2-8°C.|
For Research Use Only.