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CD6, clone: IM348, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-0061-82

Code : Z2

Additional Details : Weight : 0.01000kg

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Product Code. 15276767

  • £62.12 / 100µg


Estimated Shipment
27-02-2018
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Disclaimers:

For Research Use Only.


Description

Description

Description: The monoclonal antibody IM348 recognizes mouse CD6, a transmembrane glycoprotein of the scavenger receptor family. The extracellular domain contains three scavenger receptor cysteine rich (SRCR) domains, a transmembrane region and a cytoplasmic domain of variable length depending on the isoform. CD6 is expressed on most T lymphocytes. Expression between human and mouse is slightly different. In mice B cells in the spleen do not have CD6, while humans express it on a subset of B lymphocytes. The CD6 ligand is CD166, also known as Activated Leukocyte Cell Adhesion Molecule (ALCAM). Through interactions with CD166 or homotypic CD6-CD6, a role exists in adhesion and migration during organogenesis. The intracellular domain of CD6 is constitutively phosphorylated, suggesting a role in T cell activation and signaling. In humans depletion of CD6-expressing cells increases graft versus host tolerance in transplants.Applications Reported: This IM348 antibody has been reported for use in flow cytometric analysis, immunoblotting (WB), and immunohistology staining of frozen tissue sections.Applications Tested: This IM348 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.The IM348 antibody can also be used at <5 μg/mL in immunoblotting of non-reduced lysates.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD6
IM348
Unconjugated
Purified
IgG2a
RUO
Primary
Murine
Western Blot
0.5mg/mL
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Rat
100μg
Store at 2-8°C.
Monoclonal
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