CD56 (NCAM), eFluor 660, clone: 5tukon56, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 50-0565-80

Product Code. 15589876

Description

NCAM, as a member of the immunoglobulin superfamily of adhesion molecules is characterized by several immunoglobulin (Ig)-like domains. The extracellular part of NCAM consists of five of these Ig domains and two fibronectin type III homology regions. NCAM is encoded by a single copy gene composed of 26 exons. However, at least 20-30 distinct isoforms can be generated by alternative splicing and by posttranslational modifications, such as sialylation. During sialylation, polysialic acid (PSA) carbohydrates are attached to the extracellular part of NCAM. Through its extracellular region, NCAM mediates homophilic interactions. In addition, NCAM can also undergo heterophilic interactions by binding extracellular matrix components, such as laminin, or other cell adhesion molecules, such as integrins.

Description: This 5tukon56 monoclonal antibody reacts with human CD56, also known as Neural Cell Adhesion Molecule (NCAM). CD56 is a highly glycosylated transmembrane molecule expressed in both the developing and adult nervous system and plays a role in neurogenesis, migration, neurite extension, and the homotypic adhesion of neural cells. In the hematopoietic system, CD56 is expressed on NK cells and a subset of T cells referred to as NKT cells.For flow cytometric analysis of human CD56, we recommend clones CMSSB or TULY56.Applications Reported: This 5tukon56 antibody has been reported for use in immunohistochemical staining of formalin-fixed paraffin embedded tissue sections.Applications Tested: This 5tukon56 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded human tissue using low pH antigen retrieval at less than or equal to 10 μg/mL. It is recommmended that the antibody be carefully titrated for optimal performance in the assay of interest.eFluor™ 660 is a replacement for Alexa Fluor™ 647. eFluor™ 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.

Specifications

• Antigen: CD56 (NCAM)
• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Formulation: PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
• Gene Accession No.: P13591
• Gene Symbols: NCAM1
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 4684
• Content And Storage: Store at 2-8°C. Do not freeze. Light-sensitive material.
• Form: Liquid

• Applications: Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
• Clone: 5tukon56
• Conjugate: eFluor 660
• Gene: NCAM1
• Gene Alias: NCAM1, NCAM, CD-56, MSK39, neural cell adhesion molecule 1
• Host Species: Mouse
• Quantity: 25 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG1 κ

For Research Use Only.