Learn More
CD54 (ICAM-1) Monoclonal Antibody (eBioKAT-1 (KAT-1, KAT1)), Functional Grade, eBioscience™, Invitrogen™
Rat Monoclonal Antibody
£79.50 - £249.00
Specifications
Antigen | CD54 (ICAM-1) |
---|---|
Concentration | 1 mg/mL |
Applications | Flow Cytometry, Functional Assay, Immunohistochemistry (Frozen), Immunoprecipitation, Neutralization |
Classification | Monoclonal |
Conjugate | Functional Grade |
Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
---|---|---|---|---|---|---|---|---|---|
Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
15278477
|
Affymetrix eBioscience
16-0542-85 |
500 μg |
£249.00
500µg |
Estimated Shipment: 07-05-2024 Log in to see stock. |
Please sign in to purchase this item. Need a web account? Register with us today! | ||||
15258477
|
Affymetrix eBioscience
16-0542-81 |
50 μg |
This item is currently unavailable or has been discontinued. View the product page for possible alternatives. |
||||||
Description
Description: The eBioKAT-1 monoclonal antibody reacts with mouse CD54 (ICAM-1), which is a 95 kDa member of the immunoglobulin superfamily. CD54 is expressed at low levels on leukocytes and high endothelial venules, and expression increases in response to inflammatory cytokines. ICAM-1 binds to LFA-1 and this interaction is required for the transendothelial migration of T cells. ICAM-1-deficient mice are viable, however the migration of leukocytes to sites of inflammation is reduced leading to impaired immune and inflammatory responses. Based on the regulated expression of ICAM-1, it has been suggested that ICAM-1 may increase leukocyte extravasation at sites of inflammation, whereas the constitutively high expression of ICAM-2 mediates leukocyte traffic into non-inflamed tissue. The eBioKAT-1 monoclonal antibody recognizes a different epitope than the YN1/1.7.4 monoclonal antibody. Applications Reported: This eBioKAT-1 (KAT-1, KAT1) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistology staining of frozen tissue sections. The KAT-1 monoclonal antibody has also been reported to inhibit the in vivo ICAM-1-mediated migration of leukocytes. Applications Tested: This eBioKAT-1 (KAT-1, KAT1) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 μg per test.
ICAM-1 (CD54) is an 85-110 kDa single-chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. ICAM-1 has 7 potential N-linked glycosylation sites and shares considerable amino acid sequence homology with ICAM-3 (CD50) and ICAM-2 (CD102). ICAM-1 binds to integrins of type CD11a/CD18 (leukocyte adhesion molecule, LFA-1), or CD11b/CD18 (Mac-1) and is exploited by Rhinovirus as a receptor. ICAM-1 is expressed by activated endothelial cells and detected on epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils, with lower levels that increase upon inflammation. ICAM-1 is also detected in some carcinoma and melanoma cells. Soluble ICAM-1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes.Specifications
CD54 (ICAM-1) | |
Flow Cytometry, Functional Assay, Immunohistochemistry (Frozen), Immunoprecipitation, Neutralization | |
Functional Grade | |
Rat | |
Mouse | |
P13597 | |
IgG2a κ | |
Affinity chromatography | |
Antibody |
1 mg/mL | |
Monoclonal | |
Liquid | |
RUO | |
PBS with no preservative; pH 7.2 | |
15894 | |
Primary | |
4° C |
For Research Use Only.