missing translation for 'onlineSavingsMsg'
Learn More

CD54 (ICAM-1), Functional Grade, clone: eBioKAT-1 (KAT-1, KAT1), eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 16-0542-81

Code : Z2

Additional Details : Weight : 0.01000kg

 View more versions of this product

Product Code. 15258477

  • £62.63 / 50µg


Estimated Shipment
01-03-2018
Add to basket

Disclaimers:

For Research Use Only.


Description

Description

Description: The eBioKAT-1 monoclonal antibody reacts with mouse CD54 (ICAM-1), which is a 95kDa member of the immunoglobulin superfamily. CD54 is expressed at low levels on leukocytes and high endothelial venules, and expression increases in response to inflammatory cytokines. ICAM-1 binds to LFA-1 and this interaction is required for the transendothelial migration of T cells. ICAM-1-deficient mice are viable, however the migration of leukocytes to sites of inflammation is reduced leading to impaired immune and inflammatory responses. Based on the regulated expression of ICAM-1, it has been suggested that ICAM-1 may increase leukocyte extravasation at sites of inflammation, whereas the constitutively high expression of ICAM-2 mediates leukocyte traffic into non-inflamed tissue. The eBioKAT-1 monoclonal antibody recognizes a different epitope than the YN1/1.7.4 monoclonal antibody.Applications Reported: This eBioKAT-1 (KAT-1, KAT1) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistology staining of frozen tissue sections. The KAT-1 monoclonal antibody has also been reported to inhibit the in vivo ICAM-1-mediated migration of leukocytes.Applications Tested: This eBioKAT-1 (KAT-1, KAT1) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.Storage and handling: Use in a sterile environment.Filtration: 0.2μm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.
Specifications

Specifications

CD54 (ICAM-1)
KAT1)
Unconjugated
Purified
Rat
50μg
Store at 2-8°C.
Monoclonal
Immunoprecipitation
1mg/mL
aqueous buffer, no sodium azide
Intercellular adhesion molecule-1
IgG2a
RUO
Primary
Murine
Videos