CD54 (ICAM-1), Biotin, clone: YN1/1.7.4, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 13-0541-85

Product Code. 15279599

Description

Description: The YN1/1.7.4 monoclonal antibody reacts with mouse CD54 (InterCellular Adhesion Molecule-1, ICAM-1), a 90-110kDa transmembrane glycoprotein expressed by monocytes, lymphocytes, dendritic cells, and endothelial cells. Expression of CD54 is upregulated on activated lymphocytes. Interaction of CD54 with its ligands CD11a and CD11b is important in the inflammatory response. The YN1/1.7.4 monoclonal antibody recognizes a different epitope than the eBioKAT-1 monclonal antibody.Applications Reported: The YN1/1.7.4 antibody has been reported for use in flow cytometric analysis.Applications Tested: The YN1/1.7.4 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Filtration: 0.2μm post-manufacturing filtered.

Specifications

• Antigen: CD54 (ICAM-1)
• Classification: Monoclonal
• Concentration: 0.5mg/mL
• Formulation: PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
• Gene Accession No.: P13597
• Gene Alias: BB2, P3.58, ICAM-1, cell surface glycoprotein P3.58, intercellular adhesion molecule 1, human rhinovirus receptor, major group rhinovirus receptor
• Host Species: Rat
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Type: Antibody
• Monoclonal or Polyclonal: Monoclonal
• Target Species: Murine

• Applications: Flow Cytometry
• Clone: YN1/1.7.4
• Conjugate: Biotin
• Gene: Icam1
• Format: Conjugated
• Gene Symbols: Icam1
• Isotype: IgG2b, kappa
• Quantity: 500μg
• Storage Requirements: Store at 2-8°C. Do not freeze.
• Primary or Secondary: Primary
• Gene ID (Entrez): 15894

For Research Use Only.