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CD54 (ICAM-1), PerCP-eFluor™ 710, clone: HA58, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 46-0549-42

Code : NEW

Additional Details : Weight : 0.10000kg

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Product Code. 15324851

  • £202.80 / Pack of 100


Estimated Shipment date
from Supplier 25-05-2018
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Description

Description

Description: The HA58 monoclonal antibody reacts with human CD54 (InterCellular Adhesion Molecule-1, ICAM-1), a 90-110kDa transmembrane glycoprotein expressed by monocytes, lymphocytes and endothelial cells. Expression of CD54 is upregulated on activated lymphocytes. Interaction of CD54 with its ligand CD11a is important in the inflammatory response.Applications Reported: This HA58 antibody has been reported for use in flow cytometric analysis.Applications Tested: This HA58 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD54 (ICAM-1)
Monoclonal
5μl/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
P05362
BB2, P3.58, ICAM-1, cell surface glycoprotein P3.58, intercellular adhesion molecule 1, human rhinovirus receptor, major group rhinovirus receptor
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
HA58
PerCP-eFluor 710
ICAM1
Conjugated
ICAM1
IgG1, kappa
100 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
3383
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